Background: Osteoarthritis (OA) involves progressive cartilage degeneration, inflammatory activity and impaired joint function. Okra (Abelmoschus esculentus L.) seed oil contains unsaturated fatty acids, phenolics and tocopherols and exhibits antioxidant activity. This study evaluated whether oral okra seed oil attenuates MIA-induced knee OA in a dose-dependent manner. Methods: Thirty male Sprague–Dawley rats were observed as five groups (n=6): normal control, MIA control, MIA+diclofenac sodium (5 mg/kg/day), MIA+okra seed oil 400 mg/kg/day, and MIA+okra seed oil 800 mg/kg/day. Hindlimb grip strength was assessed at baseline and weekly for four weeks. Serum COMP, IL-1β, TNF-α and MMP-13 and Safranin O/Fast Green histology with modified Mankin scoring were included. Results: MIA produced progressive loss of grip strength and increased biochemical and histological indices of joint damage. Both okra seed oil doses improved outcomes relative to MIA control, with the 800 mg/kg/day group showing the larger effect. Diclofenac produced a strong functional and anti-inflammatory response, while high-dose okra seed oil showed a comparatively strong structural pattern. Conclusion: The observed pattern supports a dose-responsive effect of okra seed oil in experimental OA.