Ashwagandha is an ancient Ayurvedic medicinal plant. Recently it has received quite a bit of attention for its potential health benefits and its application as a replacement to synthetic drugs by herbal treatment. Commonly known colloquially as an adaptogenic plant something that might assist the body in dealing with a wide variety of stressors. Ashwagandha has many bioactive chemical constituents. It is also a variety of active constituent’s groups such as alkaloids flavonoids saponins and withanolides. It consists of the analysis of the phytochemical profile of Ashwagandha in relation to the established profile and major bio-actives reported from preliminary phytochemical and HPLC analyses. The compounds were organized into a chemical class of compounds and, chromatographically, withanolide compounds. In general, the result is consistent with the common use of Ashwagandha as a reservoir of biologically active compounds. But more evidence is needed to verify its effectiveness and safety.